During electrophoresis, an electrical field is maintained across the solution through which the particles can move freely. The instrument utilizes laser Doppler electrophoresis in order to determine the zeta potential of a given sample.
The speed of Brownian motion is affected by temperature therefore, the precise maintenance of temperature is imperative in the accurate measurement of particle size. By using this curve, the size and the size distribution for particles in a given sample can be analysed. The changes in intensity as detected by the detector are analysed with a digital auto correlator which generates a correlation function to generate a curve. A sensitive avalanche photodiode detector detects the scattering intensity at a specific angle which fluctuates with time. The Zetasizer Nano ZS uses a laser to illuminate the particles in a given sample to produce and observe the speckle patterns produced by the particles. The principle of DLS is that fine particles and molecules that are in constant random thermal motion (Brownian motion) travel at a speed relative to their size compared to the larger particles which travel slower. This instrument uses a variety of techniques that allow it to measure qualities of particles such as the particle size, zeta potential and molecular weightĪ technique known as Dynamic Light Scattering (DLS) is used for the measurement of particle sizes for a given sample. The Zetasizer system uses Non-Invasive Backscatter (NIBS) optics in the characterization of samples. The Malvern Zetasizer Nano ZS particle size analyser is an instrument can be used for the characterization of samples that contain colloids, nanoparticles and some macromolecules. Particle Size Analysis - Nano Range Malvern Zetasizer Nano ZS Particle Size Analyser
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